In many Gram-negative bacteria, the alternative sigma subunit of RNA polymerase, σ S/RpoS, remodels global gene expression to reshape the cell physiology and ensure survival under starvation and various stress conditions (the so-called general stress response). The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.Ĭompeting interests: The authors have declared that no competing interests exist. The mass spectrometry proteomics data have been deposited to the ProteomeXchange Consortium ( ) via the PRIDE partner repository with the dataset identifier PXD041489.įunding: This work was supported by the French National Agency (ANR-19-CE44-0005-01 to FN, ) and the Foundation for Medical Research (FRM FDM201806005943 to SM, ). This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.ĭata Availability: Genome sequences are available from the the NCBI SRA database ( ) with the accession number PRJNA931917. Received: JAccepted: SeptemPublished: September 15, 2023Ĭopyright: © 2023 Metaane et al. PLoS ONE 18(9):Įditor: Paulo Lee Ho, Instituto Butantan, BRAZIL (2023) Loss of CorA, the primary magnesium transporter of Salmonella, is alleviated by MgtA and PhoP-dependent compensatory mechanisms. Altogether, our data pinpoint a regulatory network, where the absence of CorA is sensed by the cell and compensated by MgtA and PhoP- dependent mechanisms.Ĭitation: Metaane S, Monteil V, Douché T, Giai Gianetto Q, Matondo M, Maufrais C, et al. These findings unravel the importance of PhoP-dependent functions in the absence of CorA when magnesium is sufficient. The abundance of several proteins involved in flagella formation, chemotaxis and secretion was lowered by the Δ corA and Δ phoP mutations in combination, but not alone. Synthetic phenotypes were observed when the Δ phoP and Δ corA mutations were combined, including a strong reduction in growth and motility, independently of the extracellular magnesium concentration. Consistently, a Δ mgtA, but not a Δ phoP, mutation slightly reduced the magnesium content of the Δ corA mutant. Under these conditions, MgtA production in the Δ corA mutant did not require PhoP. In contrast to the wild-type strain, the Δ corA mutant produced MgtA, but not MgtB, in the presence of high extracellular magnesium concentration. The Δ corA mutation conferred a competitive disadvantage to exit from stationary phase, and slightly impaired motility, but had no effect on total and free cellular magnesium contents. The σ S control of corA prompted us to evaluate the impact of CorA in stationary phase Salmonella cells, by using global and analytical proteomic analyses and physiological assays. The other two Mg 2+-transporters of Salmonella are encoded by the PhoP-activated mgtA and mgtB genes and are expressed under magnesium starvation. We have recently shown that a Δ rpoS mutation decreases the magnesium content and expression level of the housekeeping Mg 2+-transporter CorA in stationary phase Salmonella. In the foodborne pathogen Salmonella enterica serovar Typhimurium, σ S is also required for biofilm formation and virulence. In many Gram-negative bacteria, the stress sigma factor of RNA polymerase, σ S/RpoS, remodels global gene expression to reshape the physiology of stationary phase cells and ensure their survival under non-optimal growth conditions.
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